厂商 ：武汉费斯德生物科技有限公司
地址 ：湖北 武汉市主营产品 ：G蛋白活性抗体 点突变抗体 非乙酰化CAMP和C EILSA试剂盒 抗体定制服务 常规抗体
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商品详情描述
| 货号: 26902 |
| 规格: 30 ?L |
| 基因符号: Gna13 |
| 描述: Anti-Gα13-GTP Mouse Monoclonal Antibody |
| 背景介绍: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
| 免疫原: Recombinant full length active Gα13 protein |
| 经过测试的应用: IP, IHC, IF |
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推荐稀释比: IP: 1 ?g for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹:  IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004).
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Immunohistochemistry:  Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells.
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