厂商 :武汉费斯德生物科技有限公司
地址 :湖北 武汉市主营产品 :G蛋白活性抗体 点突变抗体 非乙酰化CAMP和C EILSA试剂盒 抗体定制服务 常规抗体
联系电话 :18162422833
货号: 26902 |
规格: 30 ?L |
基因符号: Gna13 |
描述: Anti-Gα13-GTP Mouse Monoclonal Antibody |
背景介绍: Heterotrimeric G proteins are essential cellular signal transducers. Gα13 is one of the G proteins that could mediate cell migration and angiogenesis. Other biochemical and physiological functions of Gα13 are being explored. |
免疫原: Recombinant full length active Gα13 protein |
经过测试的应用: IP, IHC, IF |
推荐稀释比: IP: 1 ?g for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
浓度: 1 mg/ml |
宿主: Mouse |
形式: Liquid |
克隆性: Monoclonal |
亚型: IgG2b |
纯度: Purified from ascites |
防腐剂: No |
成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
种属反应性: Anti-active Gα13 monoclonal antibody recognizes active Gα13 of vertebrates. |
储存条件: Store at -20°C. Avoid repeated freezing and thawing |
免疫沉淀/蛋白质印迹: IP/WB analysis of active Gα13 proteins. Purified Gα13 proteins were treated with GDP (lane 1) or GTPGTPγS (lane 2). After immunoprecipitation with anti-active Gα13-GTP mouse monoclonal antibody (Cat. # 26902), these proteins were separated by SDS/PAGE and western blotted with anti- Gα13 mouse monoclonal antibody (Cat. # 26004). |
Immunohistochemistry: Staining of MEF cells (treated with PDGF) with anti- Gα13-GTP monoclonal antibody showing the localization of Gα13-GTP in the leading edge of migrating cells. |