厂商 ：武汉费斯德生物科技有限公司
地址 ：湖北 武汉市主营产品 ：G蛋白活性抗体 点突变抗体 非乙酰化CAMP和C EILSA试剂盒 抗体定制服务 常规抗体
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商品详情描述
| 货号: 26408 |
| 基因符号: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
| 描述: Anti-IDH2(R172S) Mouse Monoclonal Antibody |
| 背景介绍: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172S IDH2 mutations do exist in diffusely infiltrative gliomas. |
| 免疫原: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172S, human origin. |
| 经过测试的应用: ELISA, WB, IF, IHC |
|
推荐稀释比: ELISA: 1: 1000-1: 5000 WB: 1: 100-1: 1000 IF: 1: 50-1: 100 IHC: 1: 50-1: 100 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: recognizes R172S mutant, but not wild-type IDH2 of vertebrates |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
蛋白质印迹:
Western blot analysis of recombinant IDH2(R172S) and wildtype proteins.
Purified His-tagged IDH2(R172S) (lane 1) and corresponding wild-type IDH2 protein (lane 2) were blotted with Anti-IDH2(R172S) monoclonal antibody (Cat. #26408).
免疫荧光:
Immunofluorescence of cells expressing IDH2 proteins with Anti-IDH2(R172S) antibody.
HEK293T cells were transfected with pCDNA3-GFP-IDH2(R172S) plasmid, pCDNA3-GFP-IDH2 (WT) plasmid, pCDNA3-GFP-IDH2 (R172G) plasmid, pCDNA3-GFP-IDH2 (R172K) plasmid, pCDNA3-GFP-IDH2 (R172W) plasmid or pCDNA3-GFP-IDH2 (R172H) plasmid, then fixed and stained with Anti-IDH2(R172S) monoclonal antibody (Cat. #26408)
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